Application of microlectures on medical functional experiment teaching / 中华医学教育探索杂志

Taking the design and application of microlecture on action potential of neural stem as research basis, we summarized the content, structure level and learning mode of microlecture which adapted in functional experimental teaching. Application of microlecture on the course of medical function experimental teaching, including pre-class preparation, classroom teaching and after-class review can inspire students' learning enthusiasm, improve their learning efficiency and teachers' teaching quality, so as to cultivate students' innovative thinking.

Application of microlectures on medical functional experiment teaching / 中华医学教育探索杂志

Taking the design and application of microlecture on action potential of neural stem as research basis,we summarized the content,structure level and learning mode of microlecture which adapted in functional experimental teaching.Application of microlecture on the course of medical function experimental teaching,including pre-class preparation,classroom teaching and after-class review can inspire students' learning enthusiasm,improve their learning efficiency and teachers' teaching quality,so as to cultivate students' innovative thinking.

Effects of Clinical Characteristics, Laboratory Parameters and Treatment Regimens on Prognosis of Patients with Multiple Myeloma / 中国实验血液学杂志

OBJECTIVE@#To study the effects of clinicopathological features, laboratory parameters and treatment regimens on the prognosis of patients with multiple myeloma.@*METHODS@#The clinical data of 97 patients with multiple myeloma treated with chemotherapy in Department of Hematology, the 1st Hospital of Hainan Medical College were analyzed retrospectively. The clinicopathological features (age, sex, severe anemia, light chain type, hypoproteinemia, paraplegia, renal injury, amyloidosis, complex karyotype, bone disease classification, Eastern Cooperative Oncology Group (ECOG) physical status score, complete remission, etc.), laboratory parameters (C-reactive protein, lactate dehydrogenase, blood calcium, serum β2 microglobulin, etc.), and treatment schemes (thalidomide maintenance treatment) were recorded. The patients were followed up for 1-60 months, and their total survival time were recorded. A single factor and multiple factors were used to analyze the factors affecting the total and early mortality of the patients. The survival curves were plotted by the Kaplan-Meier method and the survival analysis was carried out by Log-rank test.@*RESULTS@#Among 97 patients, 29 cases (29.90%) achieved complete remission, and 56 cases (57.73%) achineved partial remission. The total effective rate was 87.63% (85/97). At the end of the follow-up, 19 cases (19.59%) died, and 1, 3 and 5 year survival rates were 80.41%, 71.13% and 37.11% respectively. The median overall survival time was 29 (1-60) months. The results of single factor analysis showed that age, hypoproteinemia, severe anemia, paraplegia, renal injury, amyloidosis, complex karyotype, complete remission and thalidomide maintenance therapy were the factors affecting the prognosis of the patients (all P＜0.05). Further multiple factor Logistic regression analysis showed that age and hypoproteinemia, severe anemia, paraplegia, amyloidosis, complex karyotype and thalidomide maintenance treatment were factors affecting the prognosis (P＜0.05). Of the 97 patients, 8 cases died early. The results of single factor analysis showed that amyloidosis and severe anemia were risk factors for early death (all P＜0.05), and further multivariate Logistic regression analysis showed that amyloidosis was an independent risk factor for early death (P＜0.05).@*CONCLUSION@#There are many adverse factors affecting the prognosis of patients with multiple myeloma, such as age, hypoproteinemia, severe anemia, paraplegia, amyloidosis, complex karyotype and so on. The risk of early death in the patients with amyloidosis is higher, and salidomide maintenance therapy can help to prolong the life span of the patient.

Effectiveness of a text message in smoking cessation among male smokers before pregnancy in four cities of Zhejiang / 中华健康管理学杂志

Objective@#To evaluate the effectiveness of a text message for smoking cessation among male smokers before their wives' pregnancy, and to provide evidence for smoking cessation strategies.@*Methods@#A prospective observational study was conducted in four cities of Zhejiang province from April to October 2016. A total of 552 male smokers were assessed using data from structured questionnaires at baseline and were followed up at 1 and 6 months. A total of 307 participants were provided a text message (SMS) for smoking cessation intervention, along with wives' involvement in husbands' quitting programs. The intervention group was compared with the control group without intervention. Outcomes were self-reported 7-day point prevalence abstinence rates at 1-month and 6-months follow up.@*Results@#In the intervention group, the proportion of participants who self-reported to be healthy reduced from 26.4% to 15.3% at 1-month follow-up and increased to 21.8% at 6-month follow-up; in the control group, it was reduced from 19.2% to 11.4% at 1-month follow-up, and increased to 20.4% at 6-month follow-up. The 7-day point prevalence abstinence rate of the intervention group at 1 and 6 months were higher than that of the control group: at 1-month follow-up, 13.0% vs. 8.2%; at 6-month follow-up, 16.3% vs. 8.2%, respectively. Compared to the control group, the intervention group considered "determination" as the most important support (61.9% at 1 month, 60.9% at 6 months); "addiction cycle" was the main barrier (47.2% at 1 month, 48.9% at 6 months), and they were less influenced by other smokers (8.1% at 1 month, 5.2% at 6 months).@*Conclusion@#SMS smoking cessation intervention, along with wife's involvement in husband's quitting programs had a positive effect on the quitting rates.

An investigation on smoke-free environment and smoking statusat or below county-level governments / 预防医学

Objective To learn the smoke-free environment and smoking status at or below county-level governments of Zhejiang Province, and to promote a smoke-free government.Methods By multi-stage random sampling method, 4 cities were selected in Zhejiang Province, then 4 counties were selected in 4 cities, finally12 governments were selected in 4 counties.With the methods of field observation,interview and questionnaire survey,smoke-free environment and smoking behavior , tobacco related knowledge of civil servants were investigated.Results A total of 12 governments and 405 civil servants were investigated.The smoke-free environment of 12 governments were relatively well.The total smoking rate of civil servants was 17.78%, and the highest was 26.67% in Jiande City, and the lowest were 9.00% in Zhuji City.For the civil servants ,male(26.62%), aged 50-(40.74%),divorced or widowed(40.00%),graduation of technical secondary school (33.33%),cadre of section rank(57.14%),above 20 years' work(28.81%),smoking anywhere at home(50.00%), smoking in certain areas at work(22.95%)were significantly higher.The rate of cognition on the hazards of smoking was lower among the smokers than that of non-smokers (P<0.05).The rate of agreement on MPOWER tobacco control strategies and smoking bans in public places were lower among the smokers than that of non-smokers (P<0.05).Non-conditional logistic regression analysis showed that age of 30-39(OR=9.87,95%CI:1.45-67.29),50-(OR=9.87,95%CI:1.45-67.29), smoking anywhere at home(OR=12.28,95%CI:2.95-51.18),and smoking in certain areas at home(OR=6.57,95%CI:3.07-14.08) may be the risk factors about smoking behavior.Conclusion The situation of smoking control at or below county-level governments in Zhejiang Province was good, and smoking rates has declined.But tobacco related knowledge and behavior of civil servants were relatively insufficient.Aiming at the problems found in the investigation, it is necessary to strengthen education on smoking control among civil servants, and to promote a smoke-free government.

Epidemiological status of tobacco use among college students in Zhejiang province, China / 中华健康管理学杂志

Objective To understand the prevalence of tobacco use among college students in different populations by sex,age and major,and to provide evidence for smoking control and health decision.Methods A total of 4 638 college students selected in 21 colleges through stratified multi-stage cluster sampling from Zhejiang Province were surveyed by using questionnaire analysis.Indicators such as tried smoking,current smoking,and secondhand smoke exposure,etc.,were calculated by weight on age proportions from the numbers of college students in Zhejiang.Results The prevalence of tried smoking and currcut smoking among college students were 29.72％ and 10.57％,respectively.The prevalence of tried smoking was 46.52％ for men,18.25％ for women;28.57％ for undergraduate,31.53％ for junior college;the prevalence of current smoking was 18.86％ for men,3.86％ for women;8.88％ for undergraduate,13.23％ for junior college students.The snoking rate in college students increased with age,the group of ≥21 Years was the highest (37.87％,16.09％).The lowest level of smoking was medical student (21.76％,2.19％).The prevalence of secondhand smoke exposure in outdoor and indoor places,and dormitory among students were 65.49％,60.33％,and 23.66％,respectively.In the past 30 days,college students have seen tobacco advertisement (47.38％),smoking scenes (60.20％),and got tobacco related gifts (10.40％),free tobacco products (8.93％);college students have seen information about tobacco control (60.50％),cigarette pack warning labels (58.45％),and learned the tobacco knowledge in class in the past 12 months (14.03％).Conclusion Current prevalence of smoking in college students in Zhejiang province remained at a high level,the rate of secondhand smoke exposure is high and in college students there was lack of tobacco control education in class.The results indicated that the policy about tobacco control in school was not enough and the task of health promotion should be taken more arduously.

Evaluation on tobacco control strength at or below county-level governments of Zhejiang Province / 中华健康管理学杂志

Objective To evaluate the strength of tobacco control at or below county-level governments of Zhejiang Province,to explore the strategies and measures on tobacco control,and promote a smoke-free government.Methods By multistage stratified random sampling method,12 government ageucies were selected in 4 counties.The information of tobacco control strength and smoke-free environment creation was collected by interview and field observation,and the scores were calculated by using the percentage assignment method.An intercept suuvey was carried out in four counties and there were 405 civil servants under investigation.The data were analyzed by frequency statistic and correlation analysis.Results The smoking rate of civil servants was 17.8％,and the secondhand smoking rate was 61.9％;84.7％ of civil servants adnmitted the harm of smoking and 96.8％ admitted the harm of secondhand smoking.Support for forbidding the cigarette advertisements was highest and increasing the cigarette price was the weakest.The scores of smoke-free environment creation of project counties showed that the average score was 50.60,Zhuji and Luqiao got a maximum score of 57.14,Haiyan got a minimum score of 42.86.The scores of tobacco control strength showed that the average score was 82.35,Zhuji got a maximum score of 92.16,followed by Luqiao,Jiande and Haiyan counties,82.35,80.39 and 74.51 respectively.The scores of tobacco control strength,include policy,political will,capacity,and tobacco control efforts were the highest in Zhuji county.The results of correlation analysis showed that the total score of tobacco control strength had significant association with the score of smoke-free environment creation,smoking rate of civil servants,and approval rate of anti smoking in public places (P＜0.05).Conclusion The overall situation of tobacco control strength at or below county-level governments of Zhejiang province is good,but there is a relative shortage of smoke-free environment creation.Civil servants master the knowledge related to tobacco and support tobacco control policy.We should further promote the construction of smoke-free government.More effective smoking control measures should be conducted in order to raise the strength of tobacco control and finally implement the tobacco control legislation.

The application of PBL teaching in the comprehensive experiment course based on organ system / 中华医学教育探索杂志

Combining the PBL teaching method and the organ system as the core teaching method, we have carried on the teaching practice of 2 comprehensive experiments in the circulation system of hem-orrhagic shock and treatment, as well as the renal function and failure of the urinary system. The implemen-tation process has been divided into two links: preparation before class and class-room practice to improve the students' ability to study and solve the problem of scientific research.

Study on the epidemiological status of tobacco use among teenagers in Zhejiang province,China / 中华流行病学杂志

Objective To describe the prevalence of tobacco use among teenagers in different populations by sex and grades at school,and to provide evidence for the development of smoking control and health promotion programs.Methods A total of 4 797 teenagers selected from 36 schools through stratified multi-stage cluster sampling in Zhejiang province and were surveyed by using a questionnaire with 3 886 eligible ones for analysis.Indicators as tried smoking,current smoking,and secondhand smoke exposure,etc.,were calculated by weight on age proportions from the numbers of juniors in Zhejiang province.Results Prevalence rates of tried smoking and current smoking among teenagers were 10.91％ and 2.07％,respectively.7.72％ of the juniors were susceptible to future tobacco use.The prevalence rates of tried smoking were 15.65％ for boys and 5.58％ for girls,12.13％ in rural and 9.24％ in urban areas.Students from the 9th grade showed the highest rate (15.15％) while the 7th graders appeared the lowest level of susceptibility to future tobacco use (5.22％).The highest level of current smoking was seen in the group of "weekly allowance more than 30 Yuan,from parents"(4.07％).The prevalence of secondhand exposure to smoke in outdoor and indoor places,public transport,or at home were 57.60％,54.45％,46.97％,and 43.16％,respectively,among the teenagers.3 071 juniors (77.33％) saw the smoking scenes in the past 30 days but only 1 367 juniors (28.30％) were aware of the basic knowledge on tobaccos in class,in the past 12 months.Conclusions There were juniors who tried to smoke or were current smokers.The rate of secondhand exposure to smoking was high.A large number of the juniors were lack of education on knowledge related to tobacco control,in the classroom.It was important to promote and publicize the knowledge on tobacco so as to reduce the number of teenagers who were susceptible to future tobacco use.

Study on the epidemiological status of tobacco use among teenagers in Zhejiang province,China / 中华流行病学杂志

Objective To describe the prevalence of tobacco use among teenagers in different populations by sex and grades at school,and to provide evidence for the development of smoking control and health promotion programs.Methods A total of 4 797 teenagers selected from 36 schools through stratified multi-stage cluster sampling in Zhejiang province and were surveyed by using a questionnaire with 3 886 eligible ones for analysis.Indicators as tried smoking,current smoking,and secondhand smoke exposure,etc.,were calculated by weight on age proportions from the numbers of juniors in Zhejiang province.Results Prevalence rates of tried smoking and current smoking among teenagers were 10.91％ and 2.07％,respectively.7.72％ of the juniors were susceptible to future tobacco use.The prevalence rates of tried smoking were 15.65％ for boys and 5.58％ for girls,12.13％ in rural and 9.24％ in urban areas.Students from the 9th grade showed the highest rate (15.15％) while the 7th graders appeared the lowest level of susceptibility to future tobacco use (5.22％).The highest level of current smoking was seen in the group of "weekly allowance more than 30 Yuan,from parents"(4.07％).The prevalence of secondhand exposure to smoke in outdoor and indoor places,public transport,or at home were 57.60％,54.45％,46.97％,and 43.16％,respectively,among the teenagers.3 071 juniors (77.33％) saw the smoking scenes in the past 30 days but only 1 367 juniors (28.30％) were aware of the basic knowledge on tobaccos in class,in the past 12 months.Conclusions There were juniors who tried to smoke or were current smokers.The rate of secondhand exposure to smoking was high.A large number of the juniors were lack of education on knowledge related to tobacco control,in the classroom.It was important to promote and publicize the knowledge on tobacco so as to reduce the number of teenagers who were susceptible to future tobacco use.

Analysis on factors associated with intention to quit smoking in pre-pregnancy male smokers in four cities of Zhejiang / 中华健康管理学杂志

Objective To explore the determinants of willingness to quit smoking among pre-pregnancy male smokers, and to provide evidence for smoking cessation strategies. Methods An intercept survey was carried out in four cities in Zhejiang, and there were 552 pre-pregnancy male smokers from 1401 newly wedded couples under investigation. Result The pre-pregnancy male smoking rate was 39.82%, and only 6.88%of pre-pregnancy male smokers were categorized as highly nicotine-dependent. The rate of recent willingness to quit smoking was 50.54%, and reasons for quitting were as follows:a planned pregnancy (62.50%), an example for children (47.70%), and objections from family members (45.22%). The tobacco knowledge among pre-pregnancy male smokers is relatively poor, only 49.64% of smokers were aware 8 points in 11 questions; 88.75% of smoker's wives supported their husband to quit smoking. The associated factors of smoking cessation include: tobacco knowledge (OR=0.38), health self-assessment well (OR=1.90), wife's willing for husband to quit smoking (OR=2.87). Conclusion Compared to ordinary people, the proportion of intention to quit smoking was higher in pre-pregnancy male smokers. To strengthen health education and make wife initiating in husband's quitting programs would be more effective.

Research on maternal health behaviors for Yi-nationality women in poor rural areas based on the theory of reasoned action / 中华预防医学杂志

<p><b>OBJECTIVE</b>To analyze the status of maternal health behaviors and it's risk factors for Yi-nationality women in poor rural areas of Sichuan province.</p><p><b>METHODS</b>In 2012, multi-stage stratified cluster sampling method was used to select 14 villages of two poor counties in Liangshan Yi-nationality autonomous prefecture Sichuan province. At least 10 women who have infants aged 0-12 months were selected in each simple villages, a total of 284. The structured questionnaire was developed on the basis of the theory of reasoned action. Yi-nationality female college students were trained as investigators. Research indicators included prenatal care rate, hospital delivery rate, postpartum examination rate, socio-demographic characteristics, maternal health care knowledge. χ² test was used to compare the differences of above indicators among different groups. The structural equation model were used to statistical analyze.</p><p><b>RESULTS</b>In the 284 subject women, 51.7% (147/284) women owned more than 2 children, 41.6% (118/284) women were more than 30 years old, 87.3% (248/284) women were illiteracy. The prenatal care rate was 69.7% (197/284), the hospital delivery rate was 26.8% (76/284), and the postnatal check rate was 22.9% (65/284). The influence factors of maternal health behaviors included the number of children, age and education (χ² were 10.92, 13.24, 9.58; P values were 0.027, 0.004, 0.008, respectively).The structural equation model analysis results showed that the maternal health behaviors were directly or indirectly affected by subjective norms (β = 0.236, P < 0.001), women's cognition (β = 0.226, P = 0.020) and women's attitudes on maternal health behavior (β = 0.157, P = 0.001). Among subjective norms, women have high compliance to their husbands (β = 0.850, P < 0.001), their peers (β = 0.708, P < 0.001), and their mothers-in-law (β = 0.636, P < 0.001).</p><p><b>CONCLUSION</b>There were still serious problems in maternal health behaviors for Yi-nationality women in poor rural areas. The main factors included not only the women's cognition and attitudes for maternal health, but also the attitudes of important social relationships.</p>

Protective effects of 3-methyladenine on acute lung injury caused by multiple trauma in rats / 中国骨伤

<p><b>OBJECTIVE</b>To investigate the role of autophagy in acute lung injury (ALI) caused by multiple trauma in rats via pretreat with 3-methyladenine (3-MA).</p><p><b>METHODS</b>Forty-five Sprague-Dawley male rats, with age of 4 months and body weight of 250-300 g,were randomly divided into three groups. In the sham group, the rats received sphenotresia only;in the control group, the rats were made model of femur shaft fracture combined with brain injury, and treated with physiological saline by abdominal cavity at 1 hour before making model; in the 3-MA group, the rats were made model of femur shaft fracture combined with brain injury,and treated with 3-MA of 10 mg/kg by abdominal cavity at 1 hour before making model. Histologic changes and the concentration of related inflammatory factors in the damaged lung tissue were examined at 48 h after opteration, at the same time, the effect of 3-MA on the expression of LC-3 II and Beclin-1 was examined through reverse transcriptase polymerase chain reaction technique (RT-PCR).</p><p><b>RESULTS</b>Compared with sham group, LC-3 II and Beclin-1 level in control group at 48 h after operation were obviously increased (P < 0.01). Compared with control group, LC-3 II and Beclin-1 level in 3-MA group at 48 h after operation were obviously decreased (P < 0.01). Compared with sham group, the level of proinflammatory cytokines (TNF-α and IL-6) in control group obviously enhanced (P < 0.01). Compared with control group, above items in 3-MA group was obviously lower (P < 0.01). Compared with control group,the histopathological damage of lung in 3-MA group obviously reduced (P < 0.01).</p><p><b>CONCLUSION</b>Autophagy can aggravate the acute lung injury caused by fracture of shaft of femur combined with brain injuries,but 3-MA can reduce tissue damage by inhibiting the autophagy and inflammatory response.</p>

Promoting medical students' innovative ability by deepening the reform of experimental teach-ing / 中华医学教育探索杂志

Teaching level and quality of experimental curriculum were promoted markedly by deepening the reform of experimental teaching, constructing qualified experimental curriculum, opti-mizing and standardizing experimental teaching process. Medical students' innovative ability was in-tensified by constructing open innovative platform and evolving vigorous extracurricular innovative activities. Meanwhile, students' innovative consciousness, scientific research ability, ability of compre-hensive analysis and problem-solving were promoted.

Research on the application of BioImp Vet Body composition in of the second class in the experiment teaching of medical function / 中国医学装备

Objective:Through the introduction of BioImp Vet Body Composition principle and application of the second classroom experimental teaching practice in medical function, analyzer application characteristics of BioImp Vet Body Composition. Methods:We studied the distribution of body fluid and fat content determination of rats using BioImp Vet Body Composition. The device using the complex impedance measurement technique in vivo, within the second 4 k-1000 kHz scope, scanned the 256 frequency bands. We measured body water content (TBW), extracellular fluid (ECF) and intracellular fluid (ICF), in addition to the amount of fat (FFM) and fat mass (FM). Results:This measurement technique is very convenient, reliable and quick data acquisition in our second classroom teaching and laboratory. Conclusion:the measurement of BioImp Vet Body Composition can be used in the field of medical experimental teaching, has the application and characteristics of portable and feasible of the second class in the experimental teaching of Medical Function.

Molecular design, structural analysis and bactericidal activity of derivatives of antimicrobial peptide buforin II / 药学学报

A novel peptide, named BF2-X, was designed based on the structure-activity analysis of an analogue of Buforin II, named BF2-A. The BF2-X was a hybrid peptide containing the N-terminal residues 5 to 13 of BF2-A and three repeats of the C-terminal regular alpha-helical motif RLLR, and the residues 8 valine were replaced by leucine. The results of bioinformatics analysis had showed that compared with BF2-A, the helicity, positive charge, hydrophobicity rate and C-terminal amphipathy of BF2-X had remarkably enhanced. Both peptides showed a random coil structure in an aqueous solution, while displaying a typical alpha-helical structure in 50% trifluoroethanol solution (a membrane mimic condition). BF2-X exhibited higher alpha-helical contents than BF2-A in hydrophobic environment. BF2-X displayed potent antimicrobial activities against a broad spectrum of microorganisms. And BF2-X showed stronger antimicrobial activities against bacteria tested than parent peptide BF2-A. These results suggest that the alpha-helical content was directly correlated with the enhanced antibacterial activity. Both peptides had no hemolytic action on mouse erythrocyte.

Exploration on mode of designed functional experiment and improvement of innovative ability of medical students / 中华医学教育探索杂志

Designed experiment in medical functional experiment has become an important way to promote creative thinking and innovation of medical students.We applied different modes of designed experiment in undergraduates of clinical medicine and basic medicine in capital medical university,including classroom designed experiments,proposition designed experiments and free proposition designed experiments.After above reforming implements,creative thinking and innovation ability of medical students were enhanced.It also provided new ideas in future teaching reform in functional experiment.

Molecular design, structural analysis and bactericidal activity of derivatives of antimicrobial peptide buforin II / 药学学报

A novel peptide, named BF2-X, was designed based on the structure-activity analysis of an analogue of Buforin II, named BF2-A. The BF2-X was a hybrid peptide containing the N-terminal residues 5 to 13 of BF2-A and three repeats of the C-terminal regular alpha-helical motif RLLR, and the residues 8 valine were replaced by leucine. The results of bioinformatics analysis had showed that compared with BF2-A, the helicity, positive charge, hydrophobicity rate and C-terminal amphipathy of BF2-X had remarkably enhanced. Both peptides showed a random coil structure in an aqueous solution, while displaying a typical alpha-helical structure in 50% trifluoroethanol solution (a membrane mimic condition). BF2-X exhibited higher alpha-helical contents than BF2-A in hydrophobic environment. BF2-X displayed potent antimicrobial activities against a broad spectrum of microorganisms. And BF2-X showed stronger antimicrobial activities against bacteria tested than parent peptide BF2-A. These results suggest that the alpha-helical content was directly correlated with the enhanced antibacterial activity. Both peptides had no hemolytic action on mouse erythrocyte.

Application of interventional therapy on hepatocellular carcinoma with hepatic arterioportal shunts / 中国医师进修杂志

ObjectiveTo observe the clinical effect of interventional embolization on hepatocellular carcinoma(HCC) with hepatic arterioportal shunts(APS) ,in the hope of improving patients' survival quality and time. MethodsTwenty-nine patients with HCC patients and APS after a successful PVA,steel coils embolization, all patients were given routine TACE therapy. The changes of gastrointestinal bleeding,ascites,diarrhea and aminotransferase were analyzed retrospectively. ResultsNineteen cases got successful embolization in the first time[achievement ratio 65.5％(19/29)]; 3 cases got recurrence after embolization [patency ratio 10.3％(3/29)],7 cases appeared new APS[incidence ratio 24.1％(7/29),5 cases were embolized 2 times, 2 cases were embolized 3 times]. The effective rate of gastrointestinal bleeding,diarrhoea,aacites were 91.7％ (11/12), 84.6％ ( 11/13 ), 83.3％ ( 15/18 ) respectively, which had significant difference between preoperative and postoperative condition. The survival rate of 3 months,6 months, 1 year,and 2 years after operation were 96.6％ (28/29),89.7％ (26/29),65.5％ (19/29),34.5％ (10/29). Conclusion Interventional therapy is a safe and effective treatment to HCC with APS.

Oxidized low density lipoprotein and peroxisome proliferator-activated receptor α induced endogenous fibroblast growth factor 21 upregulation is protective against apoptosis in cardiac endothelial cells / 中华心血管病杂志

<p><b>OBJECTIVE</b>To investigate the effect of peroxisome proliferator-activated receptor (PPAR)α agonist bezafibrate and oxidized low density lipoprotein (ox-LDL) on fibroblast growth factor 21 (FGF21) expression and apoptosis in cardiac endothelial cells.</p><p><b>METHODS</b>The mRNA level of FGF21 was determined by real time-PCR and the protein concentration of FGF21 in culture media was detected by enzyme-linked immunosorbent assay in cultured cardiac microvascular endothelial cells (CMECs) incubated with 10, 50, 100 µg/ml ox-LDL, 50, 100 or 200 µmol/L bezafibrate alone or in combination with 100 µg/ml ox-LDL. CMECs apoptosis in various treatment groups was also determined.</p><p><b>RESULTS</b>FGF21 mRNA and protein expressions were significantly upregulated in proportion to increased ox-LDL, and 200 µmol/L bezafibrate alone also significantly upregulated FGF21 expression and CMECs apoptosis was significantly reduced in 200 µmol/L bezafibrate + 100 µg/ml ox-LDL group compared to 100 µg/ml ox-LDL group (P < 0.05).</p><p><b>CONCLUSIONS</b>Our data suggest that bezafibrate and ox-LDL induced upregulation of FGF21 might mediate the protective effect against apoptosis. Endogenous FGF21 could thus play important roles in improving the endothelial function at the early stage of atherosclerosis and slowing the development of coronary heart disease.</p>